Prevalence of Carbapenem Resistance and their Genotypic Profile among Gram-Negative Bacteria in a Tertiary Care Hospital in Western India

Background: Resistance to carbapenems due carbapenemases has been increasingly noticed worldwide. Detection of among Gram‑negative bacteria (GNB) is important for
 both clinicians and infection control practitioners. Both phenotypic molecular methods can be used for detection Carbapenemases production. Molecular although the gold standard are not routinely as they might immediately available coupled with expertise required, cost infrastructure incurred limited by number targets detected.
 Methods: Consecutive non-repeat gram negative isolates isolated from various clinical specimens intensive care unit (ICU) were included in study. Antimicrobial susceptibility testing was done on Mueller Hinton’s agar Kirby Bauer Disc diffusion method per Clinical Laboratory Standards Institute (CLSI) guidelines. Isolates resistant Meropenem further screened carbapenemase producing genes using multiplex polymerase chain reaction (PCR). The results statistically analysed.
 Result: A total 350 carbapenem resistance. Carbapenem resistance found 109 GNB. metallo‑ β‑lactamases most common followed KPC.
 Conclusion: Carbapenemase a major threat 21st century. Preventing emergence spread these pathogens through strict practices, judicious use antibiotics early timely will contribute preserving carbapenems, last resort antibiotics.